Composite

Part:BBa_K4307033:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)


J32100-AffiPmrB-PmrA-PmrC-T7 core-T3 sigma-pT3-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2035
    Illegal XbaI site found at 54
    Illegal SpeI site found at 4536
    Illegal SpeI site found at 4749
    Illegal PstI site found at 395
    Illegal PstI site found at 683
    Illegal PstI site found at 1115
    Illegal PstI site found at 4550
    Illegal PstI site found at 4735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6736
    Illegal NheI site found at 6759
    Illegal SpeI site found at 2036
    Illegal SpeI site found at 4536
    Illegal SpeI site found at 4749
    Illegal PstI site found at 395
    Illegal PstI site found at 683
    Illegal PstI site found at 1115
    Illegal PstI site found at 2050
    Illegal PstI site found at 4550
    Illegal PstI site found at 4735
    Illegal NotI site found at 2043
    Illegal NotI site found at 4543
    Illegal NotI site found at 4740
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 946
    Illegal BamHI site found at 3316
    Illegal BamHI site found at 4885
    Illegal XhoI site found at 7636
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2036
    Illegal XbaI site found at 54
    Illegal SpeI site found at 4536
    Illegal SpeI site found at 4749
    Illegal PstI site found at 395
    Illegal PstI site found at 683
    Illegal PstI site found at 1115
    Illegal PstI site found at 4550
    Illegal PstI site found at 4735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 54
    Illegal SpeI site found at 2036
    Illegal SpeI site found at 4536
    Illegal SpeI site found at 4749
    Illegal PstI site found at 395
    Illegal PstI site found at 683
    Illegal PstI site found at 1115
    Illegal PstI site found at 2050
    Illegal PstI site found at 4550
    Illegal PstI site found at 4735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6881


Design Notes

waiting


Source

waiting

References